The growing interest in the development and use of biomolecules in biomedical research and practice requires an increase in the diversity of analytical methods and their applications in academia and industry. Part of our research focuses on synthesis, function, and analysis of bioactive peptides with therapeutic relevance. Target molecules are e.g., complex cysteine-rich peptides like FXIIIa inhibitor tridegin and ion channel blocker conotoxins. The bioanalytical methods established for such molecules can be used to investigate the effects of different formulation processes on the structure and activity of proteins and peptides. In addition, we have a strong interest in the impact of ligand/protein-protein interactions such as in case of transient heme binding to proteins or modulation of G-proteins by peptidic tools.